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Microfluidic production of liposomes through liquid–liquid phase separation in ternary droplets

《化学科学与工程前沿（英文）》 2022年第16卷第6期页码 1017-1022 doi: 10.1007/s11705-021-2118-y

摘要： Liposomes, the self-assembled phospholipid vesicles, have been extensively used in various fields such as artificial cells, drug delivery systems, biosensors and cosmetics. However, current microfluidic routes to liposomes mostly rely on water-in-oil-in-water double emulsion droplets as templates, and require complex fabrication of microfluidic devices, and tedious manipulation of multiphase fluids. Here we present a simple microfluidic approach to preparing monodisperse liposomes from oil-in-water droplets. For demonstration, we used butyl acetate-water-ethanol ternary mixtures as inner phase and an aqueous solution of surfactants as outer phase to make oil-in-water droplets, which can evolve into water-in-oil-in-water double emulsion droplets by liquid–liquid phase separation of ternary mixtures. Subsequently, the resultant water-in-oil-in-water droplets underwent a dewetting transition to form separated monodisperse liposomes and residual oil droplets, with the assistance of surfactants. The method is simple, does not require complex microfluidic devices and tedious manipulation, and provides a new platform for controllable preparation of liposomes.

关键词： microfluidics liposomes ternary droplets phase separation

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叶酸靶向含硼脂质体的制备及其包封率的测定

王志会,钱林学,刘冬

《中国工程科学》 2012年第14卷第8期页码 78-81

摘要：

制备具有良好靶向性及包封率高的硼脂质体,为硼中子俘获治疗方法的研究与应用建立了一个有效的靶向给药途径。采用复乳法及薄膜—超声分散法制备叶酸靶向硼脂质体, 利用高效液相色谱法检测4—羟基苯硼酸4—Hydroxyphenylboronicacid（HBA）脂质体、2—噻吩硼酸2—thiophenylboric acid（TBA）脂质体、4—叔丁基苯硼酸4—tert-Butylphenylboronic Acid（BBA）脂质体的含量及包封率，以包封率为评价指标，采用单因素法优化脂质体的制备处方和工艺条件。筛选出HBA、TBA、BBA最优的色谱条件，分别绘制出标准曲线，结果表明在1~100 μg/mL范围内线性关系良好。HBA、TBA、BBA脂质体在最优制备处方和工艺条件下包封率分别为25.7 %、38.9 %、94.8 %。BBA 脂质体优化后的制备处方和工艺条件如下：胆固醇与磷脂质量比为 1∶1，药脂比为 1∶50，pH值为7.4，按该处方工艺制备的BBA 脂质体包封率在94.8 %。制备叶酸靶向脂质体的优选处方和制备工艺稳定可行，质量控制方法简单、准确, 包封率高。

关键词：硼中子俘获治疗脂质体叶酸靶向包封率